Publications

  • Lung-Protective Ventilation Attenuates Mechanical Injury While Hypercapnia Attenuates Biological Injury in a Rat Model of Ventilator-Associated Lung Injury. (2022).
    Nada Ismaiel, Sara Whynot, Laurette Geldenhuys, Zhaolin Xu, Arthur S. Slutsky, Valerie Chappe And Dietrich Henzler
    Frontiers in Physiology. April 21, 2022.

  • VIP reduction in the pancreas of F508del homozygous CF mice and early signs of Cystic Fibrosis Related Diabetes (CFRD). (2021).
    Anna Semaniakou, Frederic Chappe, Younes Anini, Valerie Chappe
    Journal of Cystic Fibrosis. 2021 May 24.
    https://www.sciencedirect.com/science/article/pii/S1569199321001338

  • Disrupted local innervation results in less VIP expression in CF mice tissues. (2020).
    Anna Semaniakou, Sarah Brothers, Grayson Gould, Mehrsa Zahiremani, Jamie Paton, Frederic Chappe, Audrey Li, Younes Anini, Roger P Croll, Valerie Chappe
    J Cyst Fibros. 2020 Jun 26;S1569-1993(20)30777-3. doi: 10.1016/j.jcf.2020.06.013
    https://pubmed.ncbi.nlm.nih.gov/32600901/

  • A disruption of innervation around CF tissues results in low amount of VIP before CF disease has produced damage to the lungs and small intestine. (2020).
    Anna Semaniakou, Sarah Brothers, Grayson Gould, Mehrsa Zahiremani, Jamie Paton, Frederic Chappe, Audrey Li, Younes Anini, Roger P Croll, Valerie Chappe
    J Cyst Fibros. 2020 Jun 26;S1569-1993(20)30777-3. doi: 10.1016/j.jcf.2020.06.013.
    https://www.ecfs.eu/sites/default/files/cf-research-article-files/JCF-D-19-00465R2_FINAL_Chappe_230720.pdf

  • Changes in the R‐region interactions depend on phosphorylation and contribute to PKA and PKC regulation of the cystic fibrosis transmembrane conductance regulator chloride channel (2019).
    Diogo R. Poroca, Noha Amer, Audrey Li, John W. Hanrahan, Valerie M. Chappe
    FASEB BioAdvances / Volume 2, Issue 1
    https://doi.org/10.1096/fba.2019-00053

  • An evaluation of animal models in the pathophysiology of cystic fibrosis. (2019).
    Semaniakou A, Croll R., Chappe V.
    Frontiers in Pharmacology of Ion Channels and Channelopathies. IF: 3.83
    https://doi.org/10.3389/fphar.2018.01475

  • Animal Models in the Pathophysiology of Cystic Fibrosis. (2018).
    Anna Semaniakou, Roger P Croll, Valerie Chappe>
    Front Pharmacol. 2019 Jan 4;9:1475. doi: 10.3389/fphar.2018.01475. eCollection 2018.
    https://pubmed.ncbi.nlm.nih.gov/30662403/

  • Doing what works to have a "normal" life with Cystic Fibrosis: A grounded theory (2018).
    MacDonald M, Lang A, Savage E, Chappe V, Murphy A, Grosse F, MacLean H.
    Respiratory Care.(2018), respcare.06493
    https://doi.org/10.4187/respcare.06493

  • Iron Chelation as Novel Treatment for Lung Inflammation in Cystic Fibrosis (2017).
    Maral Aali, Alexa Caldwell, Kelsey House, Juan Zhou, Valerie Chappe & Christian Lehmann.
    Medical Hypotheses. 104:86-88

  • Modeling Cystic Fibrosis Disease Progression in Patients with the Rare CFTR Mutation P67L (2017).
    Isobel E.R. MacKenzie, Valerie Paquette, Frances Gosse, Sheenagh George, Frederic Chappe, Valerie Chappe.
    Journal of Cystic Fibrosis. JCF-01466

  • CIC Channels and Transporters: Structure, Physiological Functions, and Implications in Human Chloride Channelopathies (2017).
    Diogo R. Poroca, Ryan M. Pellis and Valerie Chappe.
    Frontiers in Pharmacology. fphar.2017.00151

  • Cystic Fibrosis Transmembrane Conductance Regulator Dysfunction in VIP Knockout Mice (2014).
    Alcolado NG, Conrad DJ, Poroca D, Li M, Alshafie W, Chappe FG, Pelis RM, Anini Y, Xu Z, Hamidi S, Said S, Chappe VM.
    Am J Physiol-Cell Physiol. 307(2): C195-C207

  • VIP regulates CFTR membrane expression and function in Calu-3 cells by increasing its interaction with NHERF1 and P-ERM in a VPAC1 and PKCε-dependent manner (2014).
    Walaa Alshafie, Frederic G. Chappe, Mansong Li, Younes Anini & Valerie M. Chappe.
    Am J Physiol-Cell Physiol. 307: C107-C119

  • VIP-dependent increase in F508del-CFTR membrane localization is mediated by PKCε (2011).
    Alcolado N, Rafferty S., Chappe F., Kiberd M., Seavilleklein G. & Chappe V.
    Am J Physiol-Cell Physiol. 301(1): C53-65.

  • Profil de la Fibrose Kystique dans les communautés Acadiennes et Francophones des Maritimes (2009).
    Chappe F., McGraff K, Robinson W. & Chappe V.
    Francophonies d'Amérique. Invited Article. Issue 28:155-174

  • Rescue of Functional F508del-CFTR by VIP in the Human Nasal Epithelial Cell Line JME/CF15 (2009).
    Rafferty S, Alcolado N, Norez C, Chappe F, Pelzer S, Becq F, Chappe V.
    J. of Pharm. And Exp. Therapeutics. 331(1):2-13

  • PKC phosphorylation modulates PKA-dependent binding of the R domain to other domains of CFTR (2008).
    Seavilleklein G, Amer N, Evagelidis A, Chappe F, Hanrahan J & Chappe V.
    Am. J. of Physiol. 295: C1366-C1375

  • VIP increases apical CFTR channel density in Calu-3 cells by a PKC-dependent mechanism (2008).
    Chappe F, Loewen M E, Hanrahan J W & Chappe V.
    J. of Pharm. And Exp. Therapeutics. 327(1) :226-238.

  • Phosphorylation of CFTR by PKA promotes binding of the regulatory domain (2005).
    Chappe V, Irvine T, Liao J, Evagelidis A & Hanrahan J.
    EMBO J. 24 (15): 2730-2740.

  • A perspective on the regulation of CFTR by protein kinase C (2004).
    Hanrahan J. W., Loewen M. E. & Chappe V.
    Pediatric Pulmonology Suppl. 27: S15.2 160-161. Invited Perspective.

  • Pharmacological and signaling properties of endogenous P2y1 receptors in CFTR-expressing CHO cells (2004).
    Marcet B., Chappe V., Delmas P. & Verrier B.
    J. of Pharmacol. and Exp. Therapeutics. 309 (2): 533-539.

  • Stimulatory and inhibitory protein kinase C consensus sequences regulate the Cystic Fibrosis Transmembrane conductance Regulator (2004).
    Chappe V., Hinkson D.A., Howell L.D., Evagelisdis A., Liao J., Chang X-B., Riordan J.R. & Hanrahan J.W.
    Proc. Natl. Acad. Sci. 101(1): 390-395.

  • Negative regulation of CFTR activity by extracellular ATP involves P2Y2 receptors in CFTR-expressing CHO cells (2003).
    Marcet B., Chappe V., Delmas P., Gola M. & Verrier B.
    The Journal of Membrane Biology 194 (1): 21-32. *co-first authors.

  • Phosphorylation of protein kinase C consensus sites in NBD1 and the R domain control CFTR channel activation by PKA (2003).
    Chappe V., Hinkson D.A., Zhu T., Chang X-B., Riordan J.R. & Hanrahan J.W.
    The Journal of Physiology 548 (1): 39-52.

  • La Mucoviscidose (2000).
    Chappe V.
    123 Bio-net. Invited review.

  • Cystic Fibrosis Transmembrane conductance Regulator (CFTR) confers glibenclamide sensitivity to Outwardly Rectifying Chloride Channel (ORCC) in Hi-5 insect cells (1999).
    Julien M, Verrier B, Cerutti M, Chappe V, Gola M, Devauchelle G & Becq F.
    The Journal of Membrane Biology 168: 229-239.

  • Development of substituted Benzo[C]Quinolizinium compounds as novel activators of the Cystic Fibrosis chloride channel (1999).
    Becq F, Mettey Y, Gray MA, Galietta LJ, Dormer RL, Merten M, Metaye T, Chappe V, Marvingt-Mounir C, Zegarra-Moran O, Tarran R, Bulteau L, Derand R, Pereira MM, McPherson MA, Rogier C, Joffre M, Argent BE, Sarrouilhe D, Kammouni W, Figarella C, Verrier B, Gola M, Vierfond JM.
    The Journal of Biological Chemistry 274: 27415-27425.

  • Structural basis for specificity and potency of xanthine derivatives as activators of the CFTR chloride channel (1998).
    Chappe V, Mettey Y, Vierfond JM, Hanrahan JW, Gola M, Verrier B & Becq F.
    British Journal of Pharmacology 123: Iss 4, 683-693.
  • 2008. Protein & DNA Assays by Spectrophotometry (Software).
    Co-authors: Frederic Chappe & Valerie Chappe.
    Canadian Intellectual Property registration N°: 1070619.
    Specialized software for the analysis of protein or DNA content in samples measured by spectrophotometry.

  • 2007. Efflux Analysis Software.
    Co-authors: Frederic Chappe & Valerie Chappe.
    Canadian Intellectual Property registration N°: 1045769.
    Highly specialized software for iodide efflux samples measurement (medium scale throughput screening) with automated data acquisition and analysis.




in Peer-reviewed journals
  • Reduced VIP content accompanied by disrupted innervation in young CF mice (2019).
    Semaniakou A, Brothers S, Gould G, Chappe F, Anini Y, Croll R.P, Chappe V.
    Pediatr. Pulmonology, vol 54-Suppl2.

  • Pre-clinical evaluation of VIP-ELP PB1046 and PB1120 for the treatment of cystic fibrosis (2018).
    Chappe FG, Semaniakou A, Arnold S, Balance J, Chappe V.
    Pediatr. Pulmonology, Suppl. Vol53:2

  • Molecular mecanism of VIP corrector effect for the treatment of Cystic Fibrosis (2014).
    Valerie Chappe
    J Mol Neurosci (2014) 53 (Suppl 1):S160

  • VIP-ELP Fusion molecules PB1120 and PB1046 correct F508DEL-CFTR dysfunction (2014).
    Chappe F, Arnold S, Ballance J, Chappe V.
    Pediatric Pulmonology (49) S38.

  • NHERF1 and p-ERM mediate the stabilizing effect of Vasoactive Intestinal Peptide on membrane CFTR in Calu-3 cells (2013).
    Alshafie W, Chappe F, Li M & Chappe V.
    Pediatr. Pulmonology. Submitted.

  • VIP corrects CFTR dysfunction observed in VIP-KO mice (2013).
    Alcolado N, Conrad D, Li M, Alshafie W, Chappe F, Pelis R, Anini Y, Xu Z, Hamidi S, Said SI & Chappe V.
    Pediatr. Pulmonology. Submitted.

  • Phenotypic analysis of cystic fibrosis disease in canadian patients with the rarte mutatiom P67L (2013).
    Paquette V, Gosse F, Chappe F, Chappe V.
    Pediatr. Pulmonology. Submitted.

  • Deletion of the VIP gene IN C57BL/6 mice compromises CFTR membrane localization and induces a CF-like phenotype reversible by exogenous VIP administration (2011).
    Conrad D, Alcolado N, Anini Y, Xu Z, Chappe F, Said S, Chappe V.
    Pediatr Pulmonol. 46(S34):222.

  • Role of PKCε in the VIP-dependent increase of DF508-CFTR membrane localization (2010).
    Alcolado N., Rafferty S., Chappe F. & Chappe V.
    J. of Biochem. & Cell Biol. 53rd CSBMCB conference.

  • VIP stimulates ?F508-CFTR trafficking and membrane insertion in human nasal JME/CF15 cells (2008).
    Rafferty S. ; Alcolado N. ; Chappe F. ; Pelzer S. ; Becq F & Chappe V.
    Pediatr Pulmonol. 43(S31):218.

  • PKC stimulation by PMA increases the amount of wild-type and ΔF508-CFTR channels in the plasma membrane of BHK cells (2008).
    Rafferty S.; Chappe F.; Kiberd M.; Seavilleklein G. & Chappe V.
    Pediatr Pulmonol. 43(S31):217.

  • PKC phosphorylation is necessary for PKA-dependent binding of the R domain with the rest of CFTR (2007).
    Seavilleklein G, Evagelidis A, Amer N, Chappe F, Hanrahan J & Chappe V.
    Pediatr Pulmonol. 42(S30):204.

  • VIP increases insertion of active CFTR channels in the apical membrane of CALU-3 cells by stimulating a PKC signaling pathway (2006).
    Chappe, F.; Loewen, M. E; Hanrahan, J. W & Chappe, V.
    Pediatr Pulmonol. 41(S29):210.

  • Apical membrane insertion of CFTR is increased by protein kinase C activation (2006).
    Loewen M. E, Xanthoudakis S., Hanrahan J. W & Chappe V.
    FASEB J 20:A797.




Conference proceedings
  • Semaniakou A, Brothers S, Gould G, Chappe F, Li A, Anini Y, Croll R.P, Chappe V. (2018). Decreased VIPergic innervation in the duodenum of C57Bl/6 CF mice.
    32nd North American CF Conference, Denver, Co-USA. Poster presentation. Pediatr. Pulmonology, Suppl. Vol53:2.

  • Aali M, Caldwell A, Zhou J, Holbein B, Li A, Chappe V, Lehmann C. (2018). Iron Chelation Attenuates LPS-Induced IL-6 Release in Cystic Fibrosis Epithelial Cells.
    32nd North American CF Conference, Denver, Co-USA. Poster presentation. Pediatr. Pulmonology, Suppl. Vol53:2.

  • Poroca D, Li A, Langelaan D, Chappe V. CFTR activity involves binding of the RD to the Front Half of the channel. (2018)
    32nd North American CF Conference, Denver, Co-USA. Oral presentation. Pediatr. Pulmonology, Suppl. Vol53:2.

  • Frederic Chappe, Valerie Chappe. (2015). CFTR regulation by VIP: molecular mechanism and therapeutic potential.
    European Cystic Fibrosis Conference-Basic science

  • Alshafie W, Chappe F, Pelis R, Anini Y & Chappe V. (2013). Regulation of CFTR membrane stability by VIP: role of PKCε.
    CIHR-Young investigator forum, Toronto.

  • Paquette V & Chappe V. (2013). Comparative analysis of Cystic Fibrosis progression in patients with the rare mutation P67L.
    Cameronm Conference, Dalhousie University.

  • Alshafie W. and Chappe V. (2012). Regulation of CFTR endocytosis by VIP: role of PKCepsilon.
    Faculty of Medicine Graduate Research Day, Dalhousie University.

  • Boisvert P. & Chappe V. (2012). Determination of domain-domain interactions in the Cystic Fibrosis Transmembrane conductance Regulator (CFTR) channel under phosphorylated conditions, using co-transfected cells clones.
    Cameron Conference, Dalhousie University.

  • Alcolado N., Rafferty S., Chappe F., Pelzer S., Norez C., Becq F., Said S. and Chappe V. (2010). Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) membrane localization is regulated by Vasoactive Intestinal Peptide (VIP).
    Faculty of Medicine Graduate Research Day, Dalhousie University.

  • Conrad D., Anini Y., Alcolado N., Said S. and Chappe V. (2010). Effect of Chronic Vasoactive Intestinal Peptide Stimulation on CFTR Membrane Stability and Function in C57/BL Mouse Epithelium.
    Faculty of Medicine Graduate Research Day, Dalhousie University.

  • Alcolado N., Rafferty S, Chappe F, Pelzer S, Norez C, Becq F & Chappe V.(2009). Correcting cystic fibrosis disease causing mutation ΔF508 with the peptide hormone Vasoactive Intestinal Peptide (VIP).
    Faculty of Medicine Graduate Research Day, Dalhousie University.

  • Amer N, Seavilleklein G, Tang H, Chappe F & Chappe V. (2009). Examining the role of PKC phosphorylation sites in CFTR domain-domain interactions and channel activity.
    Faculty of Medicine Graduate Research Day, Dalhousie University.

  • Conrad D. & Chappe V. (2009). Cystic Fibrosis: P67L mutated CFTR Trafficking and Membrane Localization.
    Cameron Conference, Dalhousie University.

  • Alcolado N, Murphy M & Chappe V. (2008). Role of PKC and PKA in VIP stimulated increased membrane insertion of ΔF508-CFTR proteins.
    NSERC-USRA Research Day, Dalhousie University.

  • Amer N., Seavilleklein G., Tang H., Chappe F. & Chappe V. (2008). Identification of PKC phosphorylation sites that promote RD association with the rest of the CFTR chloride channel.
    Faculty of Medicine Graduate Research Day, Dalhousie University.

  • Rafferty S. Chappe V & Stewart D (2008). ΔF508-CFTR stability at the apical membrane of human nasal epithelial cells. Biofeedback Conference 2008.
    Acadia University, Wolfville, NS.

  • Siddiqi F. & Chappe V. (2008). Cellularl localization of the P67L mutated CFTR protein.
    Cameron Conference, Dalhousie University.

  • Alcolado N., Pelzer S. & Chappe V. (2008). Identifying G proteins involved in VIP-stimulated rescue of Cystic Fibrosis disease causing mutation: ΔF508.
    Cameron Conference, Dalhousie University.

  • Siddiqi F., Amer N., Chappe F., Robinson W. & Chappe V. (2007). Profil de la Fibrose Kystique dans les communautés Acadiennes et Francophones des Maritimes.
    2ėme forum sur la recherche en sant�. Ottawa, ON.

  • Shetty N., Chappe F. & Chappe V. (2006). Modulation of CFTR Regulatory Domain interaction by PKC phosphorylation.
    Faculty of Medicine Graduate Students Research Day, Dalhousie University.

  • Chappe V. (2005). Molecular and functional studies of epithelial ion channels and related diseases. CIHR-Institute of Genetics & Institute of Human Development, Child and Youth Health.
    New Principal Investigator Meeting. Toronto, ON.

  • Shetty N., Chappe F. & Chappe V. (2005). Role of phosphorylation in interaction of the R domain with other parts of the CFTR chloride channel.
    Faculty of Medicine Graduate Students Research Day, Dalhousie University.

  • Chappe V., Irvine T., Liao J., Evagelidis A. & Hanrahan J. W. (2005). Phosphorylation of CFTR by PKA promotes binding of the regulatory domain.
    The Physiology of Ion Transport Meeting, University of Bristol, Bristol UK.