Expertise

• Biochemistry:
• Protein assays.
• Western blots.
• Proteins solubilisation & extraction.
• Biotinylation and extraction of membrane proteins.
• Membrane protein recycling.

• Electrophysiology:
• Patch-clamp recording of ion channels: unitary and macroscopic currents recording.

• Immunology:
• Co-immunoprecipitations.
• Immuno labelling & detection by confocal microscopy.

• Histology & IHC:
• Rodent and human tissues.

• Microscopy:
• Fluorescence & Confocal microscopy.
• Determination of protein expression level and localization, co-localizations of proteins or domains.
• Measure of calcium.

• Molecular Biology:
• RNA isolation & RT-PCR.
• Site-directed mutagenesis.
• Sub-cloning & enzymatic digestions with analysis in electrophoresis.
• Plasmids amplification and purification.
• Recombinant protein labelling with molecular tags.

• Pharmacology:
• Regulation of ion channels by phosphorylation.
• Iodide efflux of chloride channels.

• Tissue culture & Cell Biology Assays:
• Mice tissue culture: trachea.
• Epithelial cells growth and polarization.
• Human epithelial cell lines: Calu-3, JME/CF15
• Immortalized rodent cell lines: BHK, CHO.
• Transient and stable expression of recombinant proteins. Several clones expressing wild-type and mutated CFTR channels with or without tags are available in the lab.
• Membrane labelling.